Wednesday, May 24, 2017

The oddest short film about chromatography you will see today



Via Twitter and C&EN editor Craig Bettenhausen, a film about chromatography I had never seen. I won't say that it's laugh-out-loud funny, but it has its moments. 

7 comments:

  1. Does anyone know if this system works any better than the more standard column chromatography?

    ReplyDelete
    Replies
    1. This is a really old technique that my graduate advisor used to claim was the best way for separation. We had one, albeit from a different manufacturer, but never used it, however when compared to an isco/biotage system, it's not quite as analytical/intuitive but does apparently use significantly less solvent. Something like the Rotachrom (similar type of centrifugal separation) I feel would be ideal on the manufacturing scale if you absolutely must chrom batches regularly.

      Delete
    2. Gosh brings back memories as used the Chromatatron(?) extensively in grad school (80s) then also bought for for first couple labs (process and med) I worked in because was great for certain types of efforts. Prior to acquisition we had often used prep TLC to isolate materials and this blew that away for ease of handling and capture of desired band(s). Probably not as effective in tight separations as column or flash but when had small amounts (5-25 mg) was hard to beat where if good profile could quickly preform multiple repeats to improve purity of target. Also one could, with thicker plates, do greater quantity preps for 100-250 mg loads as often did for cleaning up Reference Standards. We made our own plates as found worked better than those could buy which high load contained binder reducing silica load, however it required practice, skill and technique to get right as seen people place on the spinner then the silica slide off which made for a mess (and good teasing). If I was still in a lab, either process or medchem, I would want one around and would probably use it routinely when possible although once get experience with prep HPLCs having one of those around is even better all-a-round.

      Delete
  2. Monty python anyone? Link in name.

    ReplyDelete
    Replies
    1. It felt like about 3/4 Holy Grail and 1/4 Robin Hood: Men in Tights, as filtered through the pen of a pothead.

      Delete
  3. At the very end one of the monks is Mike Castle who was then a member of Congress from Delaware. He was famously beaten in the Republican primary by Tea Party candidate Christine O'Donnell when he ran for Joe Biden's vacated Senate Seat who was then trounced in the general election. At the state level, Delaware has been completely Democratic since after decades of elections in which candidates of both parties were able to win state wide elections.

    ReplyDelete
  4. Truck plows AnalTech, odor leads to HazMat situation

    http://www.metafilter.com/167178/Truck-plows-AnalTech-odor-leads-to-HazMat-situation

    ReplyDelete